- Transmission of bacteria from mothers to infants at birth is thought to set up the infant gut microbiome, but tracking the origin and persistence of the early colonizers has been a challenge.
- A new study finds that selected maternal bacteria, particularly Actinobacteria and Bacteroidia strains that are essential components of the infant microbiome, seed the gut of vaginally-born infants and expand to form a stable community.
- Selection for Actinobacteria and Bacteroidia likely occurs in the infant gut due to their ability to use human milk as a food source.
- Infant gut bacteria are intermittently replaced in later childhood by strains from family members, and birth by C-section appears to prevent maternal seeding of the infant gut.
The gut microbes of infants play an important role in the early development of the infant immune system and may have long-term health effects . These microbes are thought to be transmitted from the mother at birth [2-5]. However, studying where exactly the infant gut microbes originate and how long they persist has been a challenge.
In a new study, Professor Peer Bork and his colleagues at the European Molecular Biology Laboratory tracked the presence of maternal bacteria in the infant gut . They found that selected maternal bacteria, particularly Actinobacteria and Bacteroidia strains that are essential components of the infant microbiome, seed the gut of vaginally-born infants and expand to form a stable community. However, the researchers did not find maternal transmission at birth in infants born via C-section. In addition, the study revealed that strains from the environment, including from family members, occasionally replaced existing strains in later childhood.
Previous studies suggest that microbes from the vagina or from breast milk are unlikely to account for the majority of infant gut species [7,8]. Infant gut microbes are most likely to originate in the mother’s gut, which contains most of the same species, although they are present at different relative abundances [5,8]. Some studies have shown that microbes present in the environment can colonize the infant gut after birth . But it’s still unclear how long maternal microbes persist in the infant gut, and how the infant gut microbiome is affected by microbes from other family members and the environment.
In the new study, the researchers analyzed the genomes of gut bacteria from a cohort of family members in order to track strain transmission. They looked for rare single nucleotide variations (SNVs) shared between the gut bacterial strains of family members. SNVs have been shown to be able to track maternal transmission [8-10]. The researchers looked for rare SNVs shared exclusively between the gut bacteria of mothers and their babies, as this represented evidence of maternal transmission.
The researchers found that maternal strains were selectively transmitted to vaginally born infants and persisted in the infant gut. Strains of Actinobacteria and Bacteroidia were transmitted from the mother and persisted for at least one year. However, maternal strains of Clostridia, which are abundant in the mother’s gut microbiome, were not seen in the infant.
The researchers suggest that selection for Actinobacteria and Bacteroidia likely occurs in the infant gut and may arise due to the infants’ human milk-based diet. Actinobacteria and Bacteroidia species of bacteria are able to use human milk as a food source, and may thus gain a selective advantage over other bacteria in the gut of human milk-fed infants [11,12].
The study also showed that maternally transmitted strains were very stable during the first year of life, but later in childhood these strains were occasionally replaced by strains from the environment and from family members. Fathers appeared to account for most of the novel strains in the family environment, as they were more frequent donors of novel strains to other family members than recipients.
The researchers looked at maternal transmission in infants born via C-section and found that these infants did not show maternal transmission at birth. However, they gradually acquired maternal strains from the environment after birth. This suggests that vaginal birth may be the main transmission route for maternal gut microbes.
The researchers conclude that the transfer of gut bacteria from mothers to infants is a selective process and the maternal bacteria that colonize the infant gut persist and expand to form a stable microbiome. They suggest that the stability of maternal strains in the infant gut shows their importance relative to non-maternal strains, and hypothesize that maternal strains may have a protective effect by preventing the influx and growth of other potentially harmful strains in the infant gut. Future studies will need to further elucidate the benefits of maternal transmission and the consequences of transmission from the environment and family members.
1. Houghteling P.D., Walker W.A. Why is initial bacterial colonization of the intestine important to infants’ and children’s health? J Pediatr Gastroenterol Nutr. 2015 Mar;60(3):294-307.
2. Cho I., Blaser M.J. The human microbiome: at the interface of health and disease. Nat Rev Genet. 2012 Mar 13;13(4):260-70.
3. Funkhouser L.J., Bordenstein S.R. Mom knows best: the universality of maternal microbial transmission. PLoS Biol. 2013;11(8):e1001631.
4. Bäckhed F., Roswall J., Peng Y., Feng Q., Jia H., Kovatcheva-Datchary P., Li Y., Xia Y., Xie H., Zhong H., Khan M.T., Zhang J., Li J., Xiao L., Al-Aama J., Zhang D., Lee Y.S., Kotowska D., Colding C., Tremaroli V., Yin Y., Bergman S., Xu X., Madsen L., Kristiansen K., Dahlgren J., Wang J. Dynamics and stabilization of the human gut microbiome during the first year of life. Cell Host Microbe. 2015 May 13;17(5):690-703.
5. Dominguez-Bello M.G., Costello E.K., Contreras M., Magris M., Hidalgo G., Fierer N., Knight R. Delivery mode shapes the acquisition and structure of the initial microbiota across multiple body habitats in newborns. Proc Natl Acad Sci U S A. 2010 Jun 29;107(26):11971-5.
6. Korpela K., Costea P., Coelho L.P., Kandels-Lewis S., Willemsen G., Boomsma D.I., Segata N., Bork P. Selective maternal seeding and environment shape the human gut microbiome. Genome Res. 2018 Apr;28(4):561-8.
7. Ravel J., Gajer P., Abdo Z., Schneider G.M., Koenig S.S., McCulle S.L., Karlebach S., Gorle R., Russell J., Tacket C.O., Brotman R.M., Davis C.C., Ault K., Peralta L., Forney L.J. Vaginal microbiome of reproductive-age women. Proc Natl Acad Sci U S A. 2011 Mar 15;108 Suppl 1:4680-7.
8. Asnicar F., Manara S., Zolfo M., Truong D.T., Scholz M., Armanini F., Ferretti P., Gorfer V., Pedrotti A., Tett A., Segata N. Studying vertical microbiome transmission from mothers to infants by strain-level metagenomic profiling. mSystems. 2017 Jan 17;2(1). pii: e00164-16.
9. Yassour M., Vatanen T., Siljander H., Hämäläinen A.M., Härkönen T., Ryhänen S.J., Franzosa E.A., Vlamakis H., Huttenhower C., Gevers D., Lander E.S., Knip M.; DIABIMMUNE Study Group, Xavier R.J. Natural history of the infant gut microbiome and impact of antibiotic treatment on bacterial strain diversity and stability. Sci Transl Med. 2016 Jun 15;8(343):343ra81.
10. Nayfach S., Rodriguez-Mueller B., Garud N., Pollard K.S. An integrated metagenomics pipeline for strain profiling reveals novel patterns of bacterial transmission and biogeography. Genome Res. 2016 Nov;26(11):1612-25.
11. Sela D.A., Mills D.A. Nursing our microbiota: molecular linkages between bifidobacteria and milk oligosaccharides. Trends Microbiol. 2010 Jul;18(7):298-307.
12. Marcobal A., Barboza M., Sonnenburg E.D., Pudlo N., Martens E.C., Desai P., Lebrilla C.B., Weimer B.C., Mills D.A., German J.B., Sonnenburg J.L. Bacteroides in the infant gut consume milk oligosaccharides via mucus-utilization pathways. Cell Host Microbe. 2011 Nov 17;10(5):507-14.
Dr. Sandeep Ravindran
Freelance Science Writer