Symposium Posters 2016

Differences in the pre-weaning gut microbiomes of dairy and beef calves

Daisy Johnson, University of California, Davis, USA

Daisy Johnson1, Steve A. Frese1, and David A. Mills1 1. University of California, Davis Milk is known to both seed and feed the microbes that colonize mammalian gastrointestinal tract. In particular, milk contains complex glycans that serve a prebiotic function enriching beneficial microbes in the mammalian colon. We hypothesize that the development of the gut microbiota in bovine mammals represents an interesting model to understand the co-association of milk glycans with specific milk enriched microbial populations. Pre-weaning management of dairy and beef calves occurs... Read More...

The bacterial composition of milk is seasonally dependent and undergoes dramatic shifts in dairy processing facilities

Mary Kable, Department of Food Science and Technology, University of California Davis, USA

Mary E. Kable1, Yanin Srisengfa, Miles Laird, Jose Zaragoza, Jeremy McLeod2, Jessie Heidenreich2 and Maria L. Marco1 1. Department of Food Science and Technology, University of California Davis; 2. Hilmar Cheese Company, Hilmar, CA Background: Raw milk harbors diverse bacteria that can have significant impacts on the quality and safety of fluid milk and dairy products. Currently, the bacterial composition in raw milk after transport in tanker trucks and within commercial processing facilities is not well understood. Therefore, we set out to identify the bacterial composition of raw... Read More... Download PDF

Molecular mechanisms of human milk oligosaccharide utilization by Bifidobacterium longum subsp. longum SC596 and Bifidobacterium breve SC95

Nina Kirmiz, University of California, Davis, USA

Nina Kirmiz1, Daniel Garrido1, Daniele Lemay1, Santiago Ruiz-Moyano1, Jasmine Davis1, Sarah Totten1, Jennifer Smilowitz1, J. Bruce German1, Carlito B. Lebrilla1, and David A. Mills1 1. University of California, Davis Human milk oligosaccharides (HMO) are an abundant component of human breast milk. Bifidobacterium is often reported as a predominant genus in the microbiota of breast-fed infants and it is believed that bifidobacteria provide positive health benefits. Previous work has shown that strains of Bifidobacterium longum subsp. infantis (B. infantis) and Bifidobacterium bifidum have... Read More...

The transmission of milk-oriented microbes via the built environment: Infants and mothers as sources

Zachery Lewis, Dept. of Food Science and Technology and Foods for Health Institute, University of California, Davis, USA

Zachery T. Lewis1,2, The Baby-Associated Built Environment (BABE) Microbiome Project Team1, and David A. Mills1,2,3 1. Department of Food Science and Technology, University of California, Davis; 2. Foods for Health Institute, University of California, Davis; 3. Department of Viticulture and Enology, University of California Davis Bifidobacteria are a major component of the gut microbiome of breast-fed infants, where they consume human milk oligosaccharides. Bifidobacterial colonization of the infant gastrointestinal tract is associated with several positive health outcomes. It is... Read More...

Proteomic and RNA profiling of milk-derived exosomes from as1-casein deficient goats reveal that CSN1S1 genotype is capable to modulate the repertoire of molecules they conveyed

Patrice Martin, GABI, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France

Z. Krupova1,2, C. Pechoux1, C. Henry3, M. Dumarest1, P. Defrenaix2, and P. Martin1 1. GABI, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France; 2. EXCILONE, Elancourt, France; 3. INRA, UMR MICALIS, Plateforme d’Analyse Protéomique Paris Sud Ouest (PAPPSO), Université Paris-Saclay, Jouy-en-Josas, France Introduction: The biosynthesis pathways responsible for the production of milk are known in general outlines but the release and function of extracellular vesicles (EVs) from mammary epithelial cells (MEC) in milk is by far less known. Studying a goat model... Read More... Download PDF

SAHARA: Suppression before Amplification of Highly Abundant mRNA to optimize RNAseq for gene expression profiling

Patrice Martin, GABI, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France

C. Bevilacqua1, N. Crapart1,2, C. Hue-Beauvais1, B. Brandao1, S. Lemoine3, F. Coulpier3, P. Martin1 1. GABI, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France ; 2. EXCILONE, Elancourt, France ; 3. Plateforme de Génomique, Institut de Biologie de l’ENS, IBENS, Paris, France Corresponding author: Claudia.bevilacqua @jouy.inra.fr Introduction: Amplification of RNA obtained from microdissected cells remains a critical step that may impact RNAseq profiles, mainly regarding weakly expressed genes. The quality (RIN value) and quantity of RNA play an important role in... Read More... Download PDF

MiR-acle milk: Profiling milk-derived microRNAs using molecular barcoding technology for understanding their potential role in gastrointestinal function

Xuejing (Ivy) Men, Grasslands Research Centre, Tennent Drive, Palmerston North, New Zealand

X. Men1,2,4, M.J. McCann1,3, A. Thompson2,4, and N. Roy1,2,3,4 1. Food Nutrition & Health Team, Food & Bio-based Products, AgResearch Limited, Palmerston North, New Zealand; 2. Riddet Institute, Massey University, Palmerston North, New Zealand; 3. Gravida: National Centre for Growth and Development, Auckland, New Zealand; 4. Massey Institute of Food Science and Technology, Massey University, Palmerston North, New Zealand. Milk is a complex fluid that provides nutrition and aids postnatal development for infants, while also contributing to our overall health as we age. Recent... Read More... Download PDF

Cross-feeding of pathobionts by Bacteroides thetaiotaomicron as a possible mechanism for Necrotizing Enterocolitis

Ishita Shah, Dept. of Food Science and Technology, University of California, Davis, USA

Ishita M. Shah1, Steven A. Frese1,3, Gege Xu2, Carlito B. Lebrilla2, Juliana Maria Leite Nobrega De Moura Bell1, Daniela Barile1, and David A. Mills1 1. Department of Food Science and Technology, University of California, Davis; 2. Department of Chemistry, University of California, Davis; 3. Evolve Biosystems, Inc., Davis, CA Infants born preterm and hospitalized in the NICU are at high risk of infections. A major threat is the development of an intestinal disease termed as Necrotizing Enterocolitis (NEC), which many times requires surgical intervention. With about 600,000 infant... Read More...

Diversity and Milk Oligosaccharide Consumption of Bifidobacterium pseudocatenulatum

Guy Shani, Dept. of Food Science and Technology and Foods for Health Institute,
University of California, Davis, USA

Guy Shani1,2, Jasmine Davis2,3, Josh Cohen1,2, Sadaf Nagshbandi4, Steve Ho1 Santiago Ruiz-Moyano2, Zac Lewis1,2, Mina Popovic2,5, Steven Frese2, Juliana DeMoura Bell1,2, Daniela Barile1,2, Carlito Lebrilla2,3, David Mills1,2 1:Department of food Science and Technology, University of California, Davis 2:Foods for Health Institute, University of California, Davis 3:Department of Chemistry, University of California, Davis 4: Department of Biochemistry and Molecular Biology, University of California, Davis 5:Department of Life Sciences, University of Modena and Reggio Emilia, Modena, Italy... Read More...

The fatty acid composition of human milk is associated with infant microbial community profile

Diana Taft, Cincinnati Children’s Hospital, Cincinnati, Ohio, USA

Diana H. Taft1, Kelly A. Dingess1, Christina J. Valentine1,2, Nicholas J. Ollberding1, Doyle V. Ward3, David S. Newburg4, J. Thomas Brenna5, Robert J. McMahon2, and Ardythe L. Morrow1 1. Cincinnati Children’s Hospital, Cincinnati, Ohio; 2. Mead Johnson and Company, Glenview, IL; 3. University of Massachusetts Medical School, Worcester; 4. Boston College, MA; 5. Cornell University, Ithaca, NY Background: The fatty acid (FA) composition of human milk varies between mothers and is influenced by maternal diet and body stores. Evidence suggests that dietary FA may influence the gut... Read More...