Symposium Posters 2015

Collection of Data for the Genetic Improvement of Health Traits in Australian Dairy Cattle

Mary Abdelsayed, Faculty of Veterinary Science, The University of Sydney, Camden, Australia

M. Abdelsayed1*, P. Douglas1, J.E. Pryce2
1* Holstein Australia, Hawthorn East, VIC, 3122,

2* Department of Economic Development, Jobs, Transport and Resources, Government of Victoria Bundoora, VIC, 3083

A growing concern for dairy farmers is the improvement of dairy cow health through genetic selection. Healthy cows are more productive, easier to manage, require less intervention, have improved animal welfare and contribute to profitability by reducing production costs. However, in many countries, including Australia, industry collection of data on common health events has been sub-optimal or absent, which means there is no ability to provide breeding values and apply genetic selection for common health disorders. Also, such traits are low in heritability, meaning that although genetic... Read More... Download PDF

Effect of Probiotic Milk and Probiotic Yogurt to Counteract the Negative Effect of Obesogenic Diet

Véronique Demers-Mathieu, Dairy Science and Technology (STELA), Institute of Nutrition and Functional Foods (INAF), Department of Food Sciences and Nutrition, Laval University, Quebec City, Canada

Véronique Demers-Mathieu a,b,c*, Mélanie Le Barz a,c, Noémie Daniel a,c, Geneviève Pilon a,c, André Marette a,c, Julie Audy d, Emilie Laurin e, Ismaïl Fliss a, Daniel St-Gelais a,b

a STELA, INAF, Laval University,
b Agriculture and Food Canada (AAC), Food Research and Development Centre,
c Quebec Heart and Lung Institute (CRIUCPQ),
d Agropur Coopérative,
e Aliments Utima Inc.

Several studies have demonstrated that the gut microbiota as one of the most origin of metabolic disorders such as obesity and type 2 diabetes. Western obesogenic diet (i.e. rich in saturated/transfat and simple sugars and poor in fibers) can importantly alter gut microbiota (i. e. dysbiosis) leading to the production of pro-inflammatory cytokines and metabolic endotoxemia, therefore promoting metabolic disorders. To counteract these negative effects, probiotics (Lactobacillus, Bifidobacterium) have been used to shaping the gut microbiota (commensal bacteria) and then prevent inflammation and... Read More... Download PDF

A Role for Sim2s in Elongation Regulation of Milk Protein Gene Transcription in the Mammary Gland

Jessica Elswood, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, USA

J. Elswood, S. Pearson, M. Rijnkels, W. Porter
Department of Veterinary Integrative Biosciences, College of Veterinary Medicine, Texas A&M University

Identification of the mechanisms underlying the establishment and maintenance of functional differentiation is critical to our understanding of lactation. Genome wide analyses have recently demonstrated that regulation of differentiation occurs in large part by transcription elongation. Elongation proceeds after RNA Polymerase II (RNAPII) is released from its paused state through association with the positive transcriptional elongation factor (P-TEFb) containing catalytic enzyme, Cdk9. However, the mechanism by which regulation of these processes occur has yet to be elucidated in the mammary... Read More... Download PDF

Supplementation with Bifidobacterium Infantis in Combination with Bioactive Milk Components to Alter Microbial Composition in Children with Autism

Megan Sanctuary, Department of Nutrition, University of California, Davis, USA

Megan R. Sanctuary, MS 1,2, Jennifer T. Smilowitz, PhD 2,3, David Mills, PhD 2,3, J. Bruce German, PhD 2,3, Paul Ashwood, PhD 4,5, Kathleen Angkustsiri, MD 5,6

1Department of Nutrition, University of California at Davis, CA,
2Foods for Health Institute, University of California at Davis, CA,
3Department of Food Science and Technology, University of California at Davis, CA
4Department of Medical Microbiology and Immunology, School of Medicine, University of California at Davis, CA,
5MIND Institute, University of California at Davis, Sacramento, CA, 6Department of Pediatrics, School of Medicine, University of California at Davis, CA

A feature of the evolution of lactation is the explicit development of a symbiotic relationship between microorganisms and the mammalian host that is absolutely essential for proper physiological function. Human milk guides the colonization and composition of the infant gut microbiota more than any other environmental factor and compelling evidence is emerging that a milk-oriented microbiota (MOM) guides the development of various systemic processes (immunity, metabolism, neurological networks) and sets the infant up for a lifetime of health, including optimal neurodevelopment. The extent of... Read More... Download PDF

Human Breast Milk miRNA, Maternal Probiotic Supplementation and Atopic Dermatitis in Offspring

Melanie Rae Simpson, Department of Public Health and General Practice, Norwegian University of Science and Technology (NTNU), Trondheim, Norway

Background: Perinatal probiotic ingestion has been shown to prevent atopic dermatitis (AD) in infancy. This has been observed in a number of randomized clinical trials using a variety probiotics strains and supplementation regimes, and the strongest evidence comes from studies which include both pre- and post-natal supplementation. Results from our own study, Probiotics in Prevention of Allergy among Children in Trondheim (ProPACT), suggest that maternal supplementation alone is sufficient to prevent AD in offspring. The mechanisms behind this preventative effect are incompletely understood... Read More... Download PDF

Brucellosis as Health Hazards for Smallholder Dairy Farmers in Pakistan

Shumaila Arif, School of Veterinary and animal Science, Charles Sturt University, Australia

(1)S. Arif, (1) Hernández-Jover. (2) M, P.C. Thomson, (1) D.McGill, (3) H. M. Warriach and (1) J. Heller
1. School of Veterinary and animal Science, Charles Sturt University, Australia,
2. Faculty of Veterinary Science, University of Sydney, Australia,
3. University of Veterinary & Animal Sciences, Pakistan

Pakistan is the fifth largest milk producer globally, and small-holder dairy farms are the mainstay of most rural communities there. While some large-scale dairy operations are underway and in development in that country, the vast majority of production is via smallholder farms, typically with under ten livestock (cattle and/or buffalo), There are substantial impediments to development of these farms, in terms of animal husbandry, nutrition, and health-related factors. Further, the close contact between farmers and livestock results in many zoonotic transmissions of diseases. Brucellosis is... Read More... Download PDF

Variation in Phosphorylation Degree of Bovine Αs2-Casein

Zih-Hua Fang, INRA, France

Z.H. Fang1, 2, 3, G. Miranda1, 2, A. Delacroix-Buchet1, 2, M.H.P.W. Visker3 and P. Martin1, 2
1 AgroParisTech, UMR 1313 Génétique Animale et Biologie Intégrative, 75230 Paris, France
2 INRA, UMR 1313 Génétique Animale et Biologie Intégrative, 78350 Jouy-en-Josas, France
3 Animal Breeding and Genomics Centre, Wageningen UR Livestock Research, Wageningen 6700 AH, the Netherlands

Caseins are highly phosphorylated milk proteins interacting with calcium phosphate to form large colloidal structures called casein micelles. Phosphorylation of casein is an important post-translational modification occurring in the mammary gland under the action of kinases. However, the molecular mechanism behind is not fully understood yet. These kinases recognize the consensus motif as a glutamic acid, phosphor-serine residue, or aspartate at the position n+2 relative to the target serine or threonine residue (Ser/Thr-x-Glu/SerP/Asp; Mercier, 1981). This modification is essential for the... Read More... Download PDF

Using Whole Genome Sequences to Identify Candidate Mutations of Bovine Milk Fatty Acids and Proteins in Dairy Cattle

Presented by Zih-Hua Fang, INRA, France

Sanchez M.P. 1*, Govignon-Gion A.1*, Croiseau P. 1, Barbat A.1, Gelé M.2, Fritz S. 1,3, Miranda G. 1, Martin P.1, Boussaha M. 1, Brochard M. 2, Boichard D. 1.
1INRA, UMR1313 GABI, F78350, Jouy-en-Josas,
2 Institut de l’Elevage, F75012 Paris,
3 Allice, F75012 Paris.
* equal contributions.

In the framework of the PhenoFinlait project, 23 milk fatty acids and 6 major proteins were analyzed by genome-wide association study (GWAS) at the whole genome sequence level in three French dairy cattle breeds. Traits were estimated from Mid Infrared (MIR) spectrometry for about 600,000 test-days of 116,495 cows in first or second parity in Montbeliarde (MO), Normande (NO) and Holstein (HO) cattle breeds. More than 8600 cows (3,032 MO, 2,659 NO, and 2,954 HO) were genotyped with the Illumina 50K Beadchip®. In each breed, genotypes were first imputed to the high density (777k SNP) using HD... Read More...

Concentration and Distribution of Sialic Acid in Sow Milk during Lactation

Marefa Jahan, EH Graham Centre for Agricultural Innovation, Charles Sturt University, Wagga Wagga, Australia

M. Jahan, P. Wynn, B. Wang
EH Graham Centre for Agricultural Innovation, Charles Sturt University, Wagga Wagga, NSW, 2650, Australia.

Sialic acids (Sia), a family of 9-carbon acidic sugar molecules, are key monosaccharide units of brain gangliosides and glycoproteins and a major component of sialylated glycotope in human milk. Human milk Sia has been proposed as a bioactive compound promoting immune function, gut maturation and neurodevelopment of the newborn. Porcine milk however, has received little attention. The aims of the present study were to quantify and compare the levels of N-acetylneuraminic acid (Neu5Ac), N- glycolylneuraminic acid (Neu5Gc) and ketodeoxynonulpsonic acid (KDN) in oligosaccharide, glycoprotein and... Read More... Download PDF

Characterization of triacylglycerols containing omega-3 long-chain polyunsaturated fatty acids in bovine milk

Zhiqian Liu, AgriBio, Centre for AgriBioscience, Biosciences Research, Australia

Zhiqian Liu 1, Peter Moate 2, Vilnis Ezerniks 1, Ben Cocks 1,3 and Simone Rochfort 1,3
1 AgriBio, Centre for AgriBioscience, Biosciences Research, DEDJTR, 5 Ring Road, Bundoora, VIC 3083, Australia,
2 Farming Systems Research, Department of Economic Development, Jobs, Transport and Resources, Ellinbank Centre, 1301 Hazeldean Rd, Ellinbank, Victoria 3821, Australia,
3 School of Applied Systems Biology, La Trobe University, Bundoora , VIC 3083, Australia

Omega-3 long-chain polyunsaturated fatty acids (ω3 LC-PUFA) are low-abundance components in milk fat, but have great potential in promoting human health and development, such as prevention of cardiovascular disease, enhancing brain development in infants and anti-inflammatory activity. A comprehensive survey on triacylglycerol (TAG) molecular species that contain at least one type of ω3 LC-PUFA, namely EPA (C20:5), DHA (C22:6) and DPA (C22:5), has been conducted in this work using LC-LTQ-Orbitrap and LC-Triple Quadrupole mass spectrometry (MS) techniques. A combination of neutral loss scan,... Read More... Download PDF