Symposium Oral Presentations 2016

High-throughput milk oligosaccharide analysis using a rapid cartridge-based capillary electrophoresis instrument

Elisha Goonatileke, Dept. of Chemistry, University of California, Davis, USA

Jaime Salcedo1, Elisha Goonatileke2, Andres Guerrero3, Michael Kimzey3, Ted Haxo3, Carlito Lebrilla2, and Daniela Barile1 1. Department of Food Science and Technology, University of California, Davis; 2. Department of Chemistry, University of California, Davis; 3. ProZyme, Inc., Hayward, CA Milk oligosaccharides (OS) have received much interest for their biological roles as prebiotics, antimicrobial agents and modulators of the immune system. Milk OS are structurally complex molecules that exhibit multiple isomeric forms, and are present with a large dynamic range of concentration in... Read More... Download PDF

Glycosylated bioactives compounds in porcine milk: identification and quantification during lactation and correlation with the fecal metagenome

Jaime Salcedo, Dept. of Food Science and Technology, University of California, Davis, USA

J. Salcedo1, S.A. Frese1, A. Mudd3, R.N. Dilger3,4, M. Chichlowski5, B.M. Berg5, D.A. Mills1,2, and D. Barile1,2 1. Department of Food Science and Technology, University of California, Davis; 2. Foods for Health Institute, University of California, Davis; 3. Piglet Nutrition & Cognition Laboratory, University of Illinois, Urbana; 4. Division of Nutritional Sciences, University of Illinois, Urbana; 5. Mead Johnson Pediatric Nutrition Institute, Evansville, IN Swine are an excellent animal model because their digestive system physiopathology and anatomical structure, as well as the... Read More... Download PDF

Milk fat globules as a non-invasive source of mammary microRNA

Christine Leroux, INRA, Saint-Genès-Champanelle, France

D. Lago-Novais1,2*, K. Pawlowski1*, J.A.A. Pires1, C. Bevilacqua3, L. Mobuchon1,3, C. Boby1,Y. Faulconnier1, S. Bes1, P. Martin3, and C. Leroux1 1. INRA, VetAgroSup, Saint-Genès-Champanelle, France; 2. Universidade Federal da Bahia, Brazil; 3. INRA, AgroParisTech, Jouy-en-Josas, France *: Equal contribution of the two first authors Genomic mechanisms involved in the regulation of milk component synthesis and their secretion are not completely understood. MicroRNA (miRNA, small noncoding RNA) have been shown to influence mammary gland (MG) development and function. Samples for MG... Read More... Download PDF

Constitutive expression of microRNA-150 in mammary epithelium suppresses secretory activation and impairs de novo lipogenesis

Richard Heinz, University of Colorado Anschutz Medical Campus, Aurora, USA
Student Travel Award Recipient

Coordinated changes in protein expression govern progression from pregnancy to lactation during mammary gland (MG) postnatal development. In published profiling of miRNA expression in whole mouse MGs, many miRNAs declined precipitously between pregnancy and lactation. We postulated that the decreased expression of miRNAs in the MG during this stage of development allows translation of genes critical for milk production. To test this hypothesis, we isolated mammary epithelial cells (MECs) and performed simultaneous expression profiling of miRNA and mRNA. We identified microRNA-150 (miR-150)... Read More... Download PDF

RNA-Seq of mammary epithelial organoids from virgin and lactating glands of 15 Holstein-Friesian cows

Hannah Lyman, Genome Center, University of California, Davis, USA
Student Travel Award Recipient

Hannah S. Lyman1, Keith Bradnam2, Jessica Elswood3, Adrian Molenaar4, Kuljeet Singh3, Joanne Dobson3, Kim Oden3, Ian Korf1, Danielle G. Lemay1, and Monique Rijnkels3 1. Genome Center, University of California, Davis; 2. Institute of Cancer Research, London, England; 3. Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station; 4. AgResearch Ltd, Grassland Research Center, Palmerston North, New Zealand; 5. AgResearch Ltd, Ruakura Research Center, Hamilton, New Zealand Although the process of... Read More... Download PDF

KEYNOTE: Glycomic and glycoproteomic variations of human milk

Carlito Lebrilla, Dept. of Chemistry, University of California, Davis, USA

Glycans play key roles in diseases and in maintaining health. Despite their importance, our abilities to measure and determine precise structures have been limited because of the large structural heterogeneity and the complicated structures. Our research has focused on developing advanced separation methods, accurate and structurally informative mass spectrometry techniques and automated informatics tools that now make rapid throughput profiling of glycans possible. These methods are employed to determine the role of glycans in nutrition allowing us to observe and characterize the glycan... Read More... Download PDF

Human milk-derived EV are a major macromolecular component in breast milk with distinct bioactive properties

Bernd Stahl, Nutricia Research Centre for Specialized Nutrition, The Netherlands

Martijn J.C. van Herwijnen1, Marijke I. Zonneveld1,2, Soenita Goerdayal3, Esther N.M. Nolte–’t Hoen1, Johan Garssen2,4, Bernd Stahl4, A.F. Maarten Altelaar3, Frank A. Redegeld2, and Marca H.M. Wauben1 1. Department of Biochemistry & Cell Biology, Faculty of Veterinary Medicine, Utrecht University, The Netherlands; 2. Division of Pharmacology, Department of Pharmaceutical Sciences, Faculty of Science, Utrecht University, The Netherlands; 3. Biomolecular Mass Spectrometry and Proteomics Group, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical... Read More... Download PDF

Composition of major proteins in breast milk: High-throughput techniques for quantitative protein analysis

Michael Affolter, Nestlé Research Centre, Switzerland

Michael Affolter1, Clara L. Garcia-Rodenas1, Gerard Vinyes-Pares2, Rosemarie Jenni1, Iris Roggero1, Ornella Avanti-Nigro1, Carlos Antonio De Castro1, Ai Zhao3, Yumei Zhang3, Peiyu Wang4, Sagar K. Thakkar1, and Laurent Favre1 1. Nestlé Research Center, Nestec Ltd., Switzerland; 2. Nestlé Research Center Beijing, Nestec Ltd., People’s Republic of China; 3. Department of Nutrition and Food Hygiene, Peking University, People’s Republic of China; 4. Department of Social Medicine and Health Education, Peking University, People’s Republic of China Human breast milk protein... Read More... Download PDF

Damage to potential allergy-preventing proteins by regular heating and UVC treatment

Kasper Hettinga, Dept. of Dairy Science and Technology, Wageningen University, The Netherlands

Kasper Hettinga1, Bob Comstock2, Bethany Michele Henrick3, Erika von Mutius4, Bruce German3, and Markus Ege4 1. Food Quality & Design group, Wageningen University, The Netherlands; 2. Tamarack Biotics, Fresno, CA; 3. Department of Food Science and Technology, University of California, Davis; 4. Dr. von Hauner Children’s Hospital, Ludwig Maximilians University Munich, Germany At the IMGC-2014, data was presented on the difference in the breast milk proteome of allergic versus non-allergic mothers, which was subsequently published (Hettinga et al, 2015). The major outcome of that... Read More... Download PDF

Comparing the sensitizing capacity of raw and processed cow’s milk in a murine sensitization model for food allergy

Ton Baars, Research Institute of Organic Agriculture, Frick, Switzerland and Betty van Esch, Utrecht Institute of Pharmaceutical Sciences, Utrecht University, The Netherlands

S. Abbring1, M.A.P. Diks1, G.M. Dingjan1, T. Baars2, J. Garssen1,3, and B.C.A.M. van Esch1,3 1. Div of Pharmacology, Utrecht Institute of Pharmaceutical Sciences, Faculty of Science, The Netherlands; 2. Research Institute of Organic Agriculture (FiBL), Frick, Switzerland; 3. Nutricia Research, Utrecht, The Netherlands Epidemiological studies show that the consumption of raw milk early in life is protective against the development of allergies later in life. The effect was found to be limited to raw milk consumption and was not observed when this milk was boiled or when pasteurized... Read More... Download PDF